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This design uses the genome files linked in the Tutorial, for the first 3 loci.

CRISPR Application: Knockout
Target Organism NCBI Taxonomy ID: 511145
DownLoad GFF
Download FASTA

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Target Region:

• Limit to coding regions
• Relative to loci: gene-D8B36_RS00005 gene-D8B36_RS00010 gene-D8B36_RS00015
• Starting point reference: First CDS Starting Base
• Starting point padding: 0
• Ending point reference: First CDS Starting Base
• Ending point padding: 100

Guide RNA Design Parameters

• PAM: NGG
• Target length : 20
• Miss-matches: 4
  
• Number wanted: 3
• Scaffold sequence: GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGGTGC
• Target strand: both

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All genes had protospacers

All genes had enough protospacers

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Download:
Results (.csv)
Target Regions (.fa)
Report (.txt)
All output files (.tar.gz)

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View gRNA Design Results:
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